The trans-silencing capacity of invertedly repeated transgenes depends on their epigenetic state in tobacco
Identifieur interne : 001602 ( Main/Exploration ); précédent : 001601; suivant : 001603The trans-silencing capacity of invertedly repeated transgenes depends on their epigenetic state in tobacco
Auteurs : Miloslava Fojtova ; Annick Bleys [Belgique] ; Jana Bedr Ichova ; Helena Van Houdt [Belgique] ; Kater Ina Kr I Z Ova ; Anna Depicker [Belgique] ; Ales Kovar I KSource :
- Nucleic Acids Research [ 0305-1048 ] ; 2006.
Abstract
We studied the in trans-silencing capacities of a transgene locus that carried the neomycin phosphotransferase II reporter gene linked to the 35S promoter in an inverted repeat (IR). This transgene locus was originally posttranscriptionally silenced but switched to a transcriptionally silenced epiallele after in vitro tissue culture. Here, we show that both epialleles were strongly methylated in the coding region and IR center. However, by genomic sequencing, we found that the 1.0 kb region around the transcription start site was heavily methylated in symmetrical and non-symmetrical contexts in transcriptionally but not in posttranscriptionally silenced epilallele. Also, the posttranscriptionally silenced epiallele could trans-silence and trans-methylate homologous transgene loci irrespective of their genomic organization. We demonstrate that this in trans-silencing was accompanied by the production of small RNA molecules. On the other hand, the transcriptionally silenced variant could neither trans-silence nor trans-methylate homologous sequences, even after being in the same genetic background for generations and meiotic cycles. Interestingly, 5-aza-2-deoxy-cytidine-induced hypomethylation could partially restore signaling from the transcriptionally silenced epiallele. These results are consistent with the hypothesis that non-transcribed highly methylated IRs are poor silencers of homologous loci at non-allelic positions even across two generations and that transcription of the inverted sequences is essential for their trans-silencing potential.
Url:
DOI: 10.1093/nar/gkl180
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000E97
- to stream Istex, to step Curation: 000E88
- to stream Istex, to step Checkpoint: 001048
- to stream Main, to step Merge: 001610
- to stream Main, to step Curation: 001602
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">The trans-silencing capacity of invertedly repeated transgenes depends on their epigenetic state in tobacco</title><author><name sortKey="Fojtova, Miloslava" sort="Fojtova, Miloslava" uniqKey="Fojtova M" first="Miloslava" last="Fojtova">Miloslava Fojtova</name></author><author><name sortKey="Bleys, Annick" sort="Bleys, Annick" uniqKey="Bleys A" first="Annick" last="Bleys">Annick Bleys</name></author><author><name sortKey="Bedr Ichova, Jana" sort="Bedr Ichova, Jana" uniqKey="Bedr Ichova J" first="Jana" last="Bedr Ichova">Jana Bedr Ichova</name></author><author><name sortKey="Van Houdt, Helena" sort="Van Houdt, Helena" uniqKey="Van Houdt H" first="Helena" last="Van Houdt">Helena Van Houdt</name></author><author><name sortKey="Kr I Z Ova, Kater Ina" sort="Kr I Z Ova, Kater Ina" uniqKey="Kr I Z Ova K" first="Kater Ina" last="Kr I Z Ova">Kater Ina Kr I Z Ova</name></author><author><name sortKey="Depicker, Anna" sort="Depicker, Anna" uniqKey="Depicker A" first="Anna" last="Depicker">Anna Depicker</name></author><author><name sortKey="Kovar I K, Ales" sort="Kovar I K, Ales" uniqKey="Kovar I K A" first="Ales" last="Kovar I K">Ales Kovar I K</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:90718DCBF9989BA25653A5F90E7AADF26D5B19D7</idno><date when="2006" year="2006">2006</date><idno type="doi">10.1093/nar/gkl180</idno><idno type="url">https://api.istex.fr/document/90718DCBF9989BA25653A5F90E7AADF26D5B19D7/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000E97</idno><idno type="wicri:Area/Istex/Curation">000E88</idno><idno type="wicri:Area/Istex/Checkpoint">001048</idno><idno type="wicri:doubleKey">0305-1048:2006:Fojtova M:the:trans:silencing</idno><idno type="wicri:Area/Main/Merge">001610</idno><idno type="wicri:Area/Main/Curation">001602</idno><idno type="wicri:Area/Main/Exploration">001602</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">The trans-silencing capacity of invertedly repeated transgenes depends on their epigenetic state in tobacco</title><author><name sortKey="Fojtova, Miloslava" sort="Fojtova, Miloslava" uniqKey="Fojtova M" first="Miloslava" last="Fojtova">Miloslava Fojtova</name></author><author><name sortKey="Bleys, Annick" sort="Bleys, Annick" uniqKey="Bleys A" first="Annick" last="Bleys">Annick Bleys</name><affiliation wicri:level="1"><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University B-9052 Ghent</wicri:regionArea><wicri:noRegion>Ghent University B-9052 Ghent</wicri:noRegion></affiliation></author><author><name sortKey="Bedr Ichova, Jana" sort="Bedr Ichova, Jana" uniqKey="Bedr Ichova J" first="Jana" last="Bedr Ichova">Jana Bedr Ichova</name></author><author><name sortKey="Van Houdt, Helena" sort="Van Houdt, Helena" uniqKey="Van Houdt H" first="Helena" last="Van Houdt">Helena Van Houdt</name><affiliation wicri:level="1"><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University B-9052 Ghent</wicri:regionArea><wicri:noRegion>Ghent University B-9052 Ghent</wicri:noRegion></affiliation></author><author><name sortKey="Kr I Z Ova, Kater Ina" sort="Kr I Z Ova, Kater Ina" uniqKey="Kr I Z Ova K" first="Kater Ina" last="Kr I Z Ova">Kater Ina Kr I Z Ova</name></author><author><name sortKey="Depicker, Anna" sort="Depicker, Anna" uniqKey="Depicker A" first="Anna" last="Depicker">Anna Depicker</name><affiliation wicri:level="1"><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University B-9052 Ghent</wicri:regionArea><wicri:noRegion>Ghent University B-9052 Ghent</wicri:noRegion></affiliation></author><author><name sortKey="Kovar I K, Ales" sort="Kovar I K, Ales" uniqKey="Kovar I K A" first="Ales" last="Kovar I K">Ales Kovar I K</name></author></analytic><monogr></monogr><series><title level="j">Nucleic Acids Research</title><title level="j" type="abbrev">Nucl. Acids Res.</title><idno type="ISSN">0305-1048</idno><idno type="eISSN">1362-4962</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2006">2006</date><biblScope unit="volume">34</biblScope><biblScope unit="issue">8</biblScope><biblScope unit="page" from="2280">2280</biblScope><biblScope unit="page" to="2293">2293</biblScope></imprint><idno type="ISSN">0305-1048</idno></series><idno type="istex">90718DCBF9989BA25653A5F90E7AADF26D5B19D7</idno><idno type="DOI">10.1093/nar/gkl180</idno><idno type="local">gkl180</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0305-1048</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We studied the in trans-silencing capacities of a transgene locus that carried the neomycin phosphotransferase II reporter gene linked to the 35S promoter in an inverted repeat (IR). This transgene locus was originally posttranscriptionally silenced but switched to a transcriptionally silenced epiallele after in vitro tissue culture. Here, we show that both epialleles were strongly methylated in the coding region and IR center. However, by genomic sequencing, we found that the 1.0 kb region around the transcription start site was heavily methylated in symmetrical and non-symmetrical contexts in transcriptionally but not in posttranscriptionally silenced epilallele. Also, the posttranscriptionally silenced epiallele could trans-silence and trans-methylate homologous transgene loci irrespective of their genomic organization. We demonstrate that this in trans-silencing was accompanied by the production of small RNA molecules. On the other hand, the transcriptionally silenced variant could neither trans-silence nor trans-methylate homologous sequences, even after being in the same genetic background for generations and meiotic cycles. Interestingly, 5-aza-2-deoxy-cytidine-induced hypomethylation could partially restore signaling from the transcriptionally silenced epiallele. These results are consistent with the hypothesis that non-transcribed highly methylated IRs are poor silencers of homologous loci at non-allelic positions even across two generations and that transcription of the inverted sequences is essential for their trans-silencing potential.</div></front></TEI><affiliations><list><country><li>Belgique</li></country></list><tree><noCountry><name sortKey="Bedr Ichova, Jana" sort="Bedr Ichova, Jana" uniqKey="Bedr Ichova J" first="Jana" last="Bedr Ichova">Jana Bedr Ichova</name><name sortKey="Fojtova, Miloslava" sort="Fojtova, Miloslava" uniqKey="Fojtova M" first="Miloslava" last="Fojtova">Miloslava Fojtova</name><name sortKey="Kovar I K, Ales" sort="Kovar I K, Ales" uniqKey="Kovar I K A" first="Ales" last="Kovar I K">Ales Kovar I K</name><name sortKey="Kr I Z Ova, Kater Ina" sort="Kr I Z Ova, Kater Ina" uniqKey="Kr I Z Ova K" first="Kater Ina" last="Kr I Z Ova">Kater Ina Kr I Z Ova</name></noCountry><country name="Belgique"><noRegion><name sortKey="Bleys, Annick" sort="Bleys, Annick" uniqKey="Bleys A" first="Annick" last="Bleys">Annick Bleys</name></noRegion><name sortKey="Depicker, Anna" sort="Depicker, Anna" uniqKey="Depicker A" first="Anna" last="Depicker">Anna Depicker</name><name sortKey="Van Houdt, Helena" sort="Van Houdt, Helena" uniqKey="Van Houdt H" first="Helena" last="Van Houdt">Helena Van Houdt</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Belgique/explor/OpenAccessBelV2/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001602 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001602 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Belgique
|area= OpenAccessBelV2
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:90718DCBF9989BA25653A5F90E7AADF26D5B19D7
|texte= The trans-silencing capacity of invertedly repeated transgenes depends on their epigenetic state in tobacco
}}
| This area was generated with Dilib version V0.6.25. |  |